REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF SARS-COV2 FOR WALK-IN CLIENTS

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CITY GOVERNMENT OF ILAGAN

SERVICE NAME: REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF SARS-COV2 for Walk-in Clients.

Description of the Service: San Antonio City of Ilagan Molecular Laboratory provides RT PCR testing for COVID-19 Patients.

The procedure below describes how Molecular Laboratory processes the samples received. The results are released through the COVID-CODA website which various disease-reporting units can gain access to through their corresponding CODA account.

Office or Divisions:		MOLECULAR LABORATORY
Classification:		Simple
Type of Transactions:		G2C Government to Client
Who may avail:		All
CHECKLIST OF REQUIREMENTS		WHERE TO SECURE
Official Receipt Patients Interview Form Three (3) copies of Line list of samples (inactivated) to be tested		Cashier-SACIH Receiving area- Molecular laboratory Unboxing/extraction Room
CLIENT STEPS	AGENCY ACTION	FEES TO BE PAID	PROCESSING TIME	PERSON RESPONSIBLE
1. Client will pay at Cashier’s Office 2. Completion Patients Interview form 3. Swabbing booth 4. None 5. None 6. None 7. None 8. None 9. None 10. None 11. None 12. None	-Issue Official Receipt to walk-in clients -Signing and interviewing of patients before swabbing -Donning of Medical technology assigned and Swabbing of Walk- in Client -Register to Specimen Logbook Forwarding of specimens to Sample Handling Room via pass box -Unboxing and acceptance of specimens from reception -Labeling of vtms and linelist with Laboratory ID number (COIL -YEAR-NO.) -Sorting and Preparation of extraction and PCR worksheet -Label of MCTs and Spin column for extraction (Manual extraction and SRR) -Forwarding of labeled samples from the Specimen handling room to the Extraction room via pass box -Nucleic Acid Extraction of samples via Manual extraction (Genamplify or SRR) -Real Time PCR Detection of SARS-CoV-2 -PCR Master Mix Preparation -Template Addition -Real Time PCR run (MIC qPCR) Analysis and Interpretation of graph per samples -Sending of files to Pathologist via Email -Final Verification of results and approval for tagging and releasing. Encoding of assigned Laboratory Specimens IDs of each patient in COVID CODA website Tagging of verified laboratory results through COVID- CODA website Release of verified and approved Official Results to the Disease Reporting Units via email	2,620 for walk in patients None None None None None None None None None None None	5 minutes 5 minutes 5 minutes 2 minutes 40 minutes 30 minutes 1 hour and 30 minutes 2 hours 1 hour 1 hour and 30 minutes 1 hour 2 Hours	Aida Ilayat- Cashier Anna Joy Nicolas- Laboratory Supervisor Assigned Medical Technology on duty Assigned Medical Technology on duty Laboratory Technical staff on-duty Laboratory Technical staff on-duty Laboratory Technical staff on-duty Laboratory Technical staff on-duty Anatomic and Clinical Pathologist (Molecular Laboratory) Laboratory Technical Staff on-duty Alejandro Navarro Jr. –Laboratory Encoder Laboratory Technical Staff on-duty Alejandro Navarro Jr. –Laboratory Encoder Alejandro Navarro Jr.- Laboratory Encoder
TOTAL			10 hours and 30 minutes

Specific Condition:

In the event of problematic PCR runs due to power outage, PCR malfunction, specimen quality, reagent degradation, procedural errors etc., additional 10-12 hours shall be added to facilitate troubleshooting activities and repeat the test.

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