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REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF SARS-COV2

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CITY GOVERNMENT OF ILAGAN

SERVICE NAME: REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF SARS-COV2

Description of the Service:  San Antonio City of Ilagan Molecular Laboratory provides RT PCR testing for COVID-19 Patients.

The procedure below describes how Molecular Laboratory processes the samples received. The results are released through the COVID-CODA website which various disease-reporting units can gain access to through their corresponding CODA account. The processing time reflected below assumes that the maximum number of samples received per day is 200.

Office or Divisions: MOLECULAR LABORATORY
Classification: Simple
Type of Transactions: G2C Government to Client
Who may avail: All
CHECKLIST OF REQUIREMENTS WHERE TO SECURE
Case Investigation Form (CIF)

Annex E attachments

PBEF (Philhealth Eligibility Form)

Three (3) copies of Linelist of samples (inactivated) to be tested

 Disease Reporting Units

Disease Reporting Units

Philhealth Portal

Unboxing/extraction Room
CLIENT STEPS AGENCY ACTION FEES TO BE PAID PROCESSING TIME PERSON RESPONSIBLE
1.Handover of sample storage box and linelist with inactivated samples for COVID-19 testing.

Register to Specimen Logbook.

 -Verification of CIF

-Acceptance of CIF

-Disinfection of specimen box

-Forwarding of specimens to Sample Handling Room via passbox

 1,200 for Philhealth Claims 20 minutes Juan Lappay-

Receiving clerk

Kazelle F. Lara

Receiving clerk
2. None
  • Unboxing and acceptance of specimens from reception
  • Labeling of vtms and linelist with Laboratory ID number (COIL -YEAR-NO.)
 none 40 minutes Laboratory Technical staff on-duty
3. None
  • Sorting and Preparation of extraction and PCR worksheet
  • Label of MCTs and Spin column for extraction (Manual extraction and SRR)
  • Forwarding of labeled samples from the Specimen handling room to the Extraction room via pass box
 None 30 minutes Laboratory Technical staff on-duty
4. None
  • Nucleic Acid Extraction of samples via Manual extraction (Genamplify or SRR)
 None 1 hour and 30 minutes Laboratory Technical staff on-duty
5. None Real Time PCR Detection of SARS-CoV-2

  • PCR Master Mix Preparation
  • Template Addition
  • Real Time PCR run (MIC qPCR)
  • Analysis and Interpretation of graph per samples
  • Sending of files to Pathologist via Email
 None 2 hours Laboratory Technical staff on-duty
6. None
  • Final verification of results and approval for tagging/releasing
 None 1 hour Anatomic and Clinical Pathologist (Molecular Laboratory)
7. None
  • Encoding of assigned Laboratory specimen IDs of each patient in COVID CODA website
 None 1 hour and 30 minutes Laboratory Technical staff on-duty

Alejandro Navarro Jr. – Laboratory Encoder
8. None
  • Tagging of verified laboratory results through COVID-CODA website
 None 1 hour Laboratory Technical staff on-duty

Alejandro Navarro Jr. – Laboratory Encoder
9. None
  • Release of verified and approved Official Results to the Disease Reporting Units via Email
 None 2 hours Alejandro Navarro Jr. – Laboratory Encoder
                                                         

TOTAL:

 10 hours and 30 minutes

Specific Condition:

In the event of problematic PCR runs due to power outage, PCR malfunction, specimen quality, reagent degradation, procedural errors etc., additional 10-12 hours shall be added to facilitate troubleshooting activities and repeat the test.

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